I never tried using holding them that long but I don't envision they might keep since There exists toddler food stuff in them and it could go negative (but you can test it! Healthy Options for Animals tells us VetIQ chews use ingredients like chamomile and L-theanine to market leisure, https://injurysettlementprocess65554.ampedpages.com/helping-the-others-realize-the-advantages-of-grain-free-treats-for-dogs-61773281